Intranasal Spray Dosing Guide
This Intranasal Spray Dosing Guide provides suggested research-based protocols for
PureLife Peptides' intranasal sprays. These guidelines are based on Dr. Seeds’ peptide
research protocols and other leading industry standards. The information below is designed
to provide clarity on recommended starting dosages, frequency of use, and optimal timing
for administration to help researchers maintain consistency in their studies.
Dosages may be adjusted based on individual research needs and response to
administration. Suggested protocols below serve as a baseline, but researchers may
gradually increase dosages as required to optimize study outcomes while maintaining
appropriate safety considerations.

General Administration Guidelines
• Suggested Dosages: The doses provided below are based on established research
protocols. Dosing can be adjusted based on experimental needs.
• Administration: Administer one spray per nostril per administration to ensure even
distribution and optimal absorption.
• Storage: Keep refrigerated once opened. Store in a cool, dry place away from direct sunlight.
• Timing Consistency: For best results, administer peptides at the same time each day to
maintain consistency in research applications.
• Adaptability: Dosages may be increased gradually if necessary, depending on individual
study parameters, peptide half-life, and observed response.

Peptide-Specific Dosing Recommendations

Melanotan 2 Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: Once daily
• Best Time to Use: Morning, preferably before sun exposure
• Details: Melanin production, photoprotection, appetite modulation, libido enhancement.

TB-500 Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: Once or twice daily
• Best Time to Use: Morning and/or evening
• Details: Cellular regeneration, tissue repair, wound healing.

BPC-157 Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: Once or twice daily
• Best Time to Use: Morning and/or evening
• Details: Tissue healing, anti-inflammatory effects, protective benefits for organs.

PT-141 Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: As needed, up to 3 times per week
• Best Time to Use: 1-4 hours before activity
• Details: Sexual function and libido enhancement.

Semaglutide Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: 5 days a week (can increase to daily)
• Best Time to Use: Morning
• Details: Glucose regulation, appetite suppression, metabolic support.

Retatrutide Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: 5 days a week (can increase to daily)
• Best Time to Use: Morning
• Details: Metabolism, appetite, energy regulation.

GHK-Cu Nasal Spray
• Dosage per Administration: 2500 mcg (1 spray per nostril)
• Frequency: Once daily
• Best Time to Use: Evening
• Details: Regenerative properties, skin health, anti-inflammatory effects.

Tirzepatide Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: 5 days a week (can increase to daily)
• Best Time to Use: Morning
• Details: Weight management, glucose regulation.

NAD+ Nasal Spray
• Dosage per Administration: 10000 mcg or 10 mg (1 spray per nostril)
• Frequency: Once daily
• Best Time to Use: Morning
• Details: Cellular energy production, DNA repair, longevity research.

Selank Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: Once or twice daily

• Best Time to Use: Morning and/or afternoon
• Details: Neuroprotective, anxiolytic, cognitive-enhancing effects.

Semax Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: Once or twice daily
• Best Time to Use: Morning and/or afternoon
• Details: Cognitive enhancement, neuroprotection, brain function.

DSIP Nasal Spray
• Dosage per Administration: 500 mcg (1 spray per nostril)
• Frequency: 3-5 times per week
• Best Time to Use: Evening, 30 minutes before bed
• Details: Sleep regulation, stress modulation, endocrine function.

Repair-Recover Duo (TB-500 + BPC-157)
• Dosage per Administration: BPC 157 500 mcg
TB-500 500 mcg
• 1 spray per nostril:
• Frequency: Once or twice daily
• Best Time to Use: Morning and/or evening
• Details: Tissue repair, recovery, regeneration.

Glow Protocol (TB-500 + BPC-157 + GHK-Cu)
• Dosage per Administration:
BPC 157 500 mcg
TB-500 500 mcg
GHK-Cu 2500 mcg
• 1 spray per nostril:
• Frequency: Once or twice daily
• Best Time to Use: Evening
• Details: Regenerative properties, collagen production, skin rejuvenation.

Disclaimer:
The information provided on this platform, including suggested dosing guidelines, reference materials, and any
associated content, has not been reviewed or approved by the Food and Drug Administration (FDA) or any other
regulatory medical authority.
Peptide Marketplace does not diagnose, treat, cure, or prevent any disease or medical condition. The content shared is

based on independent research, scientific literature, and experiential insights and is intended solely for educational
and informational purposes. It should not be interpreted as medical advice or a substitute for professional healthcare
consultation.
Before implementing any protocol, individuals should consult with a licensed healthcare provider, particularly if
pregnant, nursing, taking prescription medications, or managing an existing health condition.
Some protocols are inspired by established peptide research, including but not limited to the works of Dr. Seeds
(“Peptide Protocols”) and Jay Campbell (“Optimize Your Health”).
By engaging with this information, users acknowledge their responsibility to conduct their own research and consult
qualified professionals before making any health-related decisions.

• Enhanced Regenerative Capabilities: The trio of peptides works synergistically to promote extensive tissue repair and cellular healing, potentially accelerating recovery processes beyond the capability of each peptide alone.
• Comprehensive Cellular Repair: Each peptide contributes uniquely—TB-500 improving inflammation modulation, BPC-157 enhancing healing, and GHK-CU promoting collagen production and tissue quality, making the protocol a robust model for regenerative research.
• Anti-Inflammatory and Antioxidative Effects: The combined properties may offer a potent anti-inflammatory and antioxidative effect, crucial for studies into chronic inflammation and oxidative stress.

• Chemical Formulae: TB-500: C212H350N56O78S, BPC-157: C62H98N16O22, GHK-CU: C14H24N6O4
• Molar Masses: TB-500: 4963.44 g/mol, BPC-157: 1419.535 g/mol, GHK-CU: 340.38 g/mol (Copper adds extra weight)
• CAS Numbers: TB-500: 77591-33-4, BPC-157: 137525-51-0, GHK-CU: 49557-75-7

Shelf Life: Ideally up to 18 months when stored properly.

Usage Disclaimer 

Intended Use: Research Chemical Only.

This product is strictly intended for research purposes as a chemical compound. It is designated for in vitro testing and laboratory experimentation only. All information provided on this website is for educational purposes and scientific understanding.

Legal Restrictions. By law, this product is prohibited from any form of bodily introduction into humans or animals. It is imperative that only licensed and qualified professionals handle this product in controlled laboratory settings.

Not Classified as a Drug, Food, or Cosmetic. Please note that this product is not classified as a drug, food, or cosmetic. It must not be misrepresented, misused, or mislabeled as such under any circumstances.